Mammalian Cell Culture Clarification-Hangzhou Anow Microfiltration Co.,Ltd.

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Mammalian Cell Culture Clarification

2013-11-03

Mammalian cells are cultured by various methods for the production of therapeutic and diagnostic proteins in different scales. These cells are grown in a bioreactor or fermentation vessel.
The first step of purification is to remove cells, debris and particles prior to the next step. TFF or NFF could be used in the first step. Whether to use TFF or NFF depends on batch sizes and frequencies of feeding. If the batch feeding is small or in a low frequency, NFF is much better for its cost economics. In other cases, TFF is more suitable.

Separation Goals
Clarification/Prefiltration
(Step One and Step Two)
Remove cells, cell debris and particles effectively and economically

Final Filtration
Reduce bioburden or remove bacteria prior to further purification steps

Application Requirements
One-step filtration is not enough to remove all the particles for their great number and ranges. It’s more cost-effective to use two or three steps filtration in most circumstances.

Recommendation

Filtration Step	Recommendation
Prefiltration	LPGF, LPPF
Fine filtration	LPGF
Final filtration	LPS, LPSD
Sterile venting	LPTA

Mammalian cell.png

Last Case: LVP

Next Case: No information!

Biopharmaceuticals

Food&Beverage

Medical

Laboratory

Microelectronics

Chemical & Water Treatment

Mammalian Cell Culture Clarification